TY - JOUR AU - Gaikwad, Apurva AU - Wadnerwar, Nilima AU - Deshmikh, Akhilesh PY - 2021/03/31 Y2 - 2024/03/29 TI - Comparative HPLC analysis of different samples of Tribhuvankirti Rasa JF - International Journal of Ayurvedic Medicine JA - IJAM VL - 12 IS - 1 SE - Research Articles DO - 10.47552/ijam.v12i1.1739 UR - https://www.ijam.co.in/index.php/ijam/article/view/1739 SP - 84-89 AB - <p>Background: There are different methods of <em>Vatsanabha shodhana</em> but it is not mentioned in literature that which method should be used in preparation of <em>Tribhuvankirti rasa</em> which will make the drug safer. Objectives: To acquire knowledge regarding toxic principle in self prepared and market sample of <em>Tribhuvankirti Rasa</em> with respect to different <em>shodhana</em> methods of <em>Vatsanabha</em> and to develop a method of testing toxic principle in the formulation. Material and methods: Three samples were prepared out of which two samples were prepared using different <em>shodhana</em> media and one sample was prepared using impure ingredients and three samples of <em>Tribhuvankirti Rasa </em>were purchased from market<em>.</em> Physicochemical and phytochemical analysis of the final product was conducted and the observations were compared among six samples. Results: HPLC graphs indicate that the numbers of peaks are increased in the sample prepared with <em>Godugdda shodhita Vatsanabha</em> and all the market samples in comparison with <em>Ashodhita</em> and <em>Gomutra</em> <em>shodhita</em> samples of <em>Tribhuvankirti Rasa. </em>All samples contain alkaloids, however after purification there was decrease in the concentration of detected alkaloid. Conclusion: The toxic principle (Aconitine) in market and self prepared sample of <em>Tribhuvankirti Rasa</em> has been reduced by <em>shodhana.</em> In addition, flavonoids, glycosides and anti-oxidants are found which are beneficial for health.</p> ER -