TY - JOUR AU - Chatuphale, Gajanan Diliprao AU - Chalakh, Sonali AU - Anjankar, Meghsham PY - 2021/03/31 Y2 - 2024/03/29 TI - A Comparative HPLC Quantification of Vatsanabha (Aconitum ferox Wall ex Seringe.) Root Processed in Cow's Urine and Cow's Milk JF - International Journal of Ayurvedic Medicine JA - IJAM VL - 12 IS - 1 SE - Research Articles DO - 10.47552/ijam.v12i1.1740 UR - https://www.ijam.co.in/index.php/ijam/article/view/1740 SP - 124-128 AB - <p><em>Vatsanabh mula</em> <span class="Apple-converted-space">&nbsp; </span>(<em>Aconitum<span class="Apple-converted-space">&nbsp; </span>ferox</em><span class="Apple-converted-space">&nbsp; </span>Wall<span class="Apple-converted-space">&nbsp; </span>ex<span class="Apple-converted-space">&nbsp; </span>Seringe root);<span class="Apple-converted-space">&nbsp; </span>is<span class="Apple-converted-space">&nbsp; </span>toxic<span class="Apple-converted-space">&nbsp; </span>due to its chief active principle an alkaloid named as<span class="Apple-converted-space">&nbsp; </span>Aconitine.<span class="Apple-converted-space">&nbsp; </span>According to Ayurveda and modern science; it is included in one of the most poisonous plants known till today to mankind. Despite it is used widely in Ayurvedic treatment in various diseases.<span class="Apple-converted-space">&nbsp; </span>So its use in medicinal preparation should be done only after proper processing termed as <em>Shodhan</em> (purification) procedures. These processings are done in specific media. <em>Shodhan</em> procedures enhance therapeutic properties of <em>Vatsanabha</em>, reduce its toxicity &amp; convert it into medicine. In Ayurvedic literature, media like <em>Gomutra</em> (Cow’s urine), <em>Godugdha </em>(Cow’s milk) and <em>Ajadugdha</em> (Goat’s milk) has been mentioned for Shodhan procedures of Vatsanabha. This study focuses on effect of two <em>Shodhan</em> procedures of <em>Vatsanabha Mula</em> by using <em>Gomutra</em> (cow’s urine) and <em>Godugdha</em> (cow’s milk) on HPLC quantification of Aconitine while the raw roots were taken as control. Changes in HPLC chromatogram confirm the effect of <em>Shodhan</em> procedure on <em>Vatsanabha</em>. In HPLC analysis, peaks were observed at retention time 30.1. Areas under the curve were less in the samples after purification with cow’s urine compared to purification with cow’s milk, indicating denaturation of some component after purification. Hence, Vatsanabha mula purified by <em>Gomutra</em> (cow’s urine) may be regarded as better method of purification as far as toxic alkaloids are concerned.<span class="Apple-converted-space">&nbsp;</span></p> ER -