Development and Validation of HPTLC Method for Determination of Vasicine in Polyherbal Cough Syrup

  • Unmesh Keshwar
  • Soumit Pimplapure
  • Neeraja Sabnis
  • Dhurde S S
  • Shrikhande B K
Keywords: HPTLC, vasicine, polyherbal cough syrup, Adhatoda vasica


A new and simple HPTLC method was developed and validated for the quantitative estimation of biologically active compound vasicine in polyherbal Cough Syrup. TLC aluminium plates precoated with silica gel 60F-254 (0.2 mm thickness) were used. The linear ascending development was carried out in twin trough glass chamber saturated with mobile phase Ethyl acetate: methanol: ammonia (8.0 : 2.0 : 0.2 v/v/v) and densitometric determination  was carried out by TLC scanner (CAMAG) at 254 nm in reflectance/absorbance mode. The Rf value of vasicine was found to be 0.54 ± 0.03. Linearity was found to be in the concentration range of 200 ng to 1600 ng. The linear regression data for the calibration plots showed a good linear relationship with r2=0.99 for vasicine. According to the ICH guideline the method was validate for accuracy, precision, recovery, robustness and ruggedness. The vasicine content quantified from polyherbal formulation (Cough Syrup) was found well within limits. The proposed method is accurate, precise, reproducible, and can be adopted for routine analysis of vasicine from polyherbal cough syrup by HPTLC
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