Phytochemical Screening and HPTLC study of Padina tetrastromatica (Hauck)

  • Subhash R Yende
  • Sumit K Arora
  • Vipinchandra B Pande
  • Keshav S Moharir
  • Abhay M Ittadwar
Keywords: Padina tetrastromatica, Brown seaweed, Marine microalgae, Phytochemical Screening, Phytoconstituents, HPTLC fingerprint

Abstract

The marine ecology is diverse with innumerable types of natural substances, both of plant and animal origin. Padina tetrastromatica (PT) (Hauck) is a brown algae belonging to the order Dictyotales, found in coastal region. The objective of present investigation was to evaluate phytochemical profile of extracts of PT. The air dried plant material was defatted and extracted successively with solvents of increasing polarity. Incumbent study was performed with standard qualitative phytochemical tests and HPTLC fingerprint analysis using CAMAG HPTLC system. The results showed the presence of phytoconstituents like sterols, terpenoids, flavonoids, glycosides, alkaloids and carbohydrates. Furthermore, components present in extracts were resolved in best possible solvent system by HPTLC. The chloroform extract of PT displayed eight peaks, in which those with Rf values 0.28 and 0.72 were more predominant. Whereas ethanol extract of PT exhibited nine peaks, in which maximum Rf value was found to be 0.82. In conclusion, the data of this study provide useful guide and suitability for investigation of biological activity of the plant according to the phytochemical groups observed. However, further work is needed to standardize the above chemical constituents in comparison with biomarker and this result can also be measured along with the other data for setting up the standards to this plant.

Author Biographies

Subhash R Yende

Assistant Professor, Pharmacology Dept, Gurunanak College of Pharmacy, Nari, Nagpur, Maharashtra. India

Sumit K Arora

Assistant Professor, Pharmacognosy and Phytochemistry Dept, Gurunanak College of Pharmacy, Nari, Nagpur, Maharashtra. India

Vipinchandra B Pande

Assistant Professor, Pharmacology Dept, Gurunanak College of Pharmacy, Nari, Nagpur, Maharashtra. India

Keshav S Moharir

Assistant Professor, Pharmaceutics Department, Gurunanak College of Pharmacy, Nari, Nagpur, Maharashtra. India

Abhay M Ittadwar

Principal, Pharmacognosy and Phytochemistry Department, Gurunanak College of Pharmacy, Nari, Nagpur, Maharashtra. India

Published
2020-07-03
Section
Research Articles