Rapid UV/Visible Spectrophotometric Method for Hesperidin Estimation in Pharmaceutical Dosage Forms and Proniosomes

Abstract

A sensitive accurate UV/Visible spectrophotometric method was developed to determine hesperidin concentration through simple detection at low cost using rapid analysis. The analytical system reached ICH guideline standard validation which delivered both precision and accuracy results. The defined solution of methanol and phosphate buffer at pH 6.8 (30:70) established the best conditions for drug analysis. A method for determining maximum absorption wavelength demonstrated a value of 285 nm with its linear relationship represented by the equation y = 0.253x + 0.0138. The method displayed linear performance from 6-30 µg/ml with an R² value of 0.9987 which verifies its precise functioning. The analytical method achieved ICH guideline compliance for linearity, precision, specificity, robustness, ruggedness, accuracy and the determination of limit of detection (LOD-0.25 µg/ml) and limit of quantification (LOQ-0.78 µg/ml). Analysis of Hesperidin in  proniosomes through the  coacervation  phase separation method was achieved successfully using UV/Visible spectrophotometry. The validation results showed that this method stands out for its high selectivity in addition to its precise and reproducible approach which makes it appropriate for hesperidin quality control in pharmaceutical monitoring of both bulk substances and formulated preparations. Through proniosomal formulation hesperidin gains higher stability along with improved delivery capabilities when applied topically. The validated approach provides medical laboratories with a both pragmatic and precise method to analyze hesperidin content in pharmaceutical settings.