Comparative HPTLC Fingerprinting Study of Guduchi Kwatha prepared by Using Different Techniques

Abstract

Kwatha Kalpana is the basic dosage form and standard of its secondary dosage forms are depended upon it. There are various challenges in preparation of Kwatha Kalpana in terms of particle size, temperature, proportion of water etc. In order to optimize the process of Kwatha the study was planned to assess the quality by HPTLC. For this, Guduchi (Tinospora Cordifolia Linn) is selected as Kwatha drug in its fresh form. Kwatha was prepared by classical method using three different proportion of water i.e. 16 parts, 8 parts and 4 parts and reduced to 1/8^th ,1/4^th and 1/4^th part respectively. In other alternative techniques, time-based techniques were used by 1hour,2 hours,3hours and 4 hours boiling. Soxhlet extraction method was been used at three different temperatures i.e. 60⁰, 80⁰ and 100⁰C and pressure cooker technique has been used at two different boiling duration i.e. 30 minutes and 60 minutes. Comparative evaluation of HPTLC fingerprinting of all these techniques were done. Conventional method of Guduchi Kwatha which used four parts of water observed maximum number of phyto-constituents. In alternative methods, higher spots were noted in Kwatha prepared after four hours of boiling and in the Soxhlet extraction method at 60⁰C. Also, the Pressure cooker method showed highest spots after 60 minutes of boiling. Presence of common R_f values in all the studied samples of Kwatha suggest that there are some stable phyto-constituents which have no impact of types of processing.