In-vitro antidiabetic, Hepatoprotective activities and HPTLC finger print profile of Azadirachta indica flower

Abstract

As per Siddha literature Azadirachta indica A.Juss relieves nausea, dry mouth, loss of taste, vomiting, prolonged pain and stomach worms; the infusion /decoction of neem flower improves the strength and cures ulcer. Objective: The core objective of the current evaluation is to study the antidiabetic activity against α-amylase and α-glucosidase, carbon tetrachloride induced hepato-protective activity from the ethanolic extract of Azadirachta indica and to study HPTLC of ethanolic extract as a quality regulatory parameter of the authentic drug. Methods: The in-vitro antidiabetic activity was assessed by measuring the inhibition of α-glucosidase and α-amylase. The 3,5-dinitrosalicylic acid method was used for the α-amylase inhibition assay, and α- glucosidase from Saccharomyces cerevisiae of enzyme inhibition assay, with minor modifications made to the standard drug acarbose of each activity. In -vitro experimental study involved administration of CCl₄ to goat liver homogenate in in-vitro to induce hepatotoxicity. Experimental protocol involved 6 experimental groups each containing goat liver homogenate. Results: The extract exhibited a substantial level of anti-diabetic activity when related with standards. As the concentration of neem flower increased, there was a concomitant increase in the α-amylase inhibition activity. The result was significant as the A. indica showed 39 % inhibitory activity with 200 μg concentration as compared to 36 % inhibitory activity exhibited by acarbose for the same concentration. Conclusion The extract revealed statistically significant decline in protein levels, liver biomarkers like alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase in extract exposed group as compared to control group.