Quantification of Gallic Acid, HPTLC, GC-MS Profiling and Anti-inflammatory Potential of Fermented Traditional Formulation “Punarnavadyaristha”

Abstract

Background: Punarnavadyaristha (PA), is a polyherbal fermented traditional formulation therapeutically known as anti-inflammatory in the classical text of Charak Samhita. However, there is no scientific evidence about its anti-inflammatory potential in the published literature. Objective: The present study was performed to validate the potential of PA against inflammation. Along with physicochemical parameters, the quantification of gallic acid and GC-MS profiling were performed to reveal the composition of PA. Materials and methods: GC-MS profiling of PA was performed to confirm the presence of bioactive compounds using the NIST library database. Quantitative HPTLC studies were performed for the quantification of gallic acid.  The presence of reactive oxygen was assessed by DPPH assay.PA was assessed preclinically for acute oral toxicity and carrageenan (acute) and formalin (sub-acute) induced edema. Results and Discussion: The GC-MS profile of PA demonstrates the presence of 67 bioactive compounds. HPTLC studies confirm the presence of gallic acid in the formulation (4.2 %). It also shows free radical scavenging activity of 47% by DPPH assay. No acute toxicity was seen in rats up to 10.0 mL/kg dose of formulation.PA (in-house) formulation at the dose of (4 mL/kg) showed inhibition of formalin-induced paw edema by 74% and carrageenan-induced paw edema by 29%. Conclusion: The effectiveness of Punarvadyaristha on inflammatory disorder, safety, and antioxidant potential is authenticated by these studies. Standardization parameters also comply Ayurvedic Pharmacopoeia of India.