Phytochemical Profile, in vitro Antioxidant Potential and HPTLC Fingerprinting of Acalypha indica Linn. Leaves

Authors

  • Anil Badnale Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur. India.
  • Prakash Itankar Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur. India.
  • Satyendra Prasad Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur. India.
  • Nandaji Chalak Traditional healers, Vidarbha Region, Gadchiroli India.
  • Suhas Dhaswadikar Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur. India.
  • Satish Meshram Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur. India.
  • Ashwini Ghagare Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur. India.
  • Yogesh Nikam Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur. India.

DOI:

https://doi.org/10.47552/ijam.v16iS2.6165

Keywords:

HPTLC, Standardization, Antioxidant, Acalypha indica L.

Abstract

The present study aims to standardize and evaluate bioactive profiling of Acalypha indica L. leaves through pharmacognostic parameters, phytochemical screening and High-Performance Thin Layer Chromatography (HPTLC) analysis. Comprehensive standardization was carried out using physicochemical constants, including moisture content, total ash, acid-insoluble ash, and extractive values, by pharmacopoeial guidelines. Macroscopic and Microscopic analyses confirmed key diagnostic features of leaves for identification and quality control profiling. Preliminary phytochemical screening of the ethanolic extract revealed the presence of bioactive constituents such as flavonoids, alkaloids, tannins, glycosides, and phenolic compounds. Antioxidant activity was assessed using in vitro assays including DPPH, ABTS, Superoxide, Hydroxyl radical, Nitric Oxide scavenging, and CUPRAC methods. The extract exhibited significant free radical scavenging potential, particularly in the CUPRAC and Superoxide assays. HPTLC analysis showed the best separation of bands at different retention factors (Rf) when using a solvent system of Toluene: Ethyl acetate: Glacial acetic acid in the ratio of (7:2:1 v/v/v). The quantitative estimation revealed that 10 mg of the ethanolic extract of Acalypha. indica L. contained 13.90 µg of kaempferol.

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Published

2025-07-21

How to Cite

Badnale, A., Itankar, P., Prasad, S., Chalak, N., Dhaswadikar, S., Meshram, S., … Nikam, Y. (2025). Phytochemical Profile, in vitro Antioxidant Potential and HPTLC Fingerprinting of Acalypha indica Linn. Leaves. International Journal of Ayurvedic Medicine, 16(S2), 84–91. https://doi.org/10.47552/ijam.v16iS2.6165

Issue

Vol. 16 No. S2 (2025): Natural to Synthetic: The Convergence of Traditional Medicine to Modern Medicine 2025

Section

Research Articles

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