High-performance Thin Layer Chromatographic quantification of hesperidin and naringenin from Citrus sinensis peel extract

Authors

  • Ashwini Ghagare Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur - 440033, Maharashtra. India.
  • Prakash Itankar Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur - 440033, Maharashtra. India.
  • Satyendra Prasad Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur - 440033, Maharashtra. India.
  • Satish Meshram Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur - 440033, Maharashtra. India.
  • Anil Badnale Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur - 440033, Maharashtra. India.

DOI:

https://doi.org/10.47552/ijam.v16iS2.6167

Keywords:

Orange peel extract, HPTLC, Hesperidin, Naringenin, Densitometry

Abstract

Orange peel is a member of Citrus sinensis, the Rutaceae family, and is widely used for its nutritional properties. Orange peels are the waste part of the fruit and contain various therapeutic molecules. Hesperidin and naringenin are two flavonoids present in orange peel. The present study depicted the quantification of high-performance thin layer chromatography of orange peel extract using hesperidin and naringenin. The HPTLC densitometric analysis of the ethanolic extract of orange peel was carried out using a CAMAG HPTLC system, Linomat 5 applicator, and Vision CAT software. The results were obtained in the form of chromatograms scanned at 254 & 366 nm. The Rf values of hesperidin were 0.5 with mobile phase Ethyl acetate: Methanol: Water (15:04:01) and 25.93 nanograms in 1 mg of orange peel extract. Naringenin was quantified at 254 & 366 nm with Rf value 0.6 & mobile phase used was Toluene: Ethyl acetate: Formic acid (12:8:1.6v/v/v), and 14.92 nanograms of naringenin were found in 1 mg of orange peel extract.

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Published

2025-07-21

How to Cite

Ghagare, A., Itankar, P., Prasad, S., Meshram, S., & Badnale, A. (2025). High-performance Thin Layer Chromatographic quantification of hesperidin and naringenin from Citrus sinensis peel extract. International Journal of Ayurvedic Medicine, 16(S2), 98–102. https://doi.org/10.47552/ijam.v16iS2.6167

Issue

Vol. 16 No. S2 (2025): Natural to Synthetic: The Convergence of Traditional Medicine to Modern Medicine 2025

Section

Research Articles

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