Evaluation of Anti-Adipogenic and wound healing properties of Coix lachryma & Hordeum vulgare employing Human Mesenchymal Stem Cell model

Abstract

Obesity, characterized by excessive accumulation of adipose tissue, is a burgeoning epidemic linked to various comorbidities including type 2 diabetes, cardiovascular diseases, and certain cancers. The role of natural compounds in regulating adipocyte differentiation has garnered attention as a potential therapeutic strategy for obesity. Natural products offer a rich reservoir of bioactive compounds with therapeutic potential. Coix lachryma -jobi (Job's Tears) and Hordeum vulgare (Barley) are two traditional cereals recognized for their diverse health benefits, including anti-inflammatory, antioxidant, and immunomodulatory properties. This explore a comprehensive experimental framework to evaluate the anti-adipogenic and wound healing capabilities of extracts from Coix lachryma  and Hordeum vulgare using a human mesenchymal stem cell (hMSC) model. Dental Pulp Stem cells (DPSCs) were isolated and culture in cell culture media.  DPSCs were characterized for surface marker CD70, CD105, CD73, CD45, CD34 and HLA-DR. Cytotoxicity of Coix lachryma  & Hordeum vulgare were performed using different concentration, on DPSCs. Effect of both extracts on adipogenic differentiation of the DPSCs was observed. DPSCs expressed CD90, CD73, and CD105 stem cell surface marker and negative for CD34, CD45 and HLAD –DR. Coix lachryma  & Hordeum vulgare did not show any signs of cytotoxicity and wound healing prosperities either in alone or in  combination. Combination of Coix lachryma  & Hordeum vulgare inhibits the adipogenic differentiation in DPSCs. Our results demonstrate that Combination of extracts of Coix lachryma  & Hordeum vulgare are capable of modulating adipogenic pathways, presenting them as promising candidates for obesity management.