Analytical Profiling and Phytochemical Evaluation of Devdarvyadi Dhoomavarti using HPTLC and GCMS

Abstract

Dhoomapana (medicated smoking) is a fumigation technique mentioned in Ayurveda for ENT and respiratory disorders. Devadarvyadi Dhooma Varti containing Devadaru, Bala & Jatamansi is one such traditional Ayurvedic formulation mentioned in Bhavaprakasha which is used for Dhoomapana (medicated smoking) therapy, primarily indicated for disorders of the head, neck & respiratory system. Despite its therapeutic importance, advanced phytochemical characterization of this formulation remains insufficiently explored. As limited analytical studies are available on Devdarvyadi Dhoomavarti, establishment of its analytical profile and phytochemical fingerprint becomes important for standardization and quality control. The formulation was prepared following this classical reference using authenticated raw drugs. Gas Chromatography Mass Spectrometry (GCMS) and High Performance Thin Layer Chromatography (HPTLC) of the formulation were done to analyze its phytoconstituents along with volatile oil content and fume carbon content. The GCMS analysis revealed the presence of various bioactive compounds. These include sesquiterpenes, phenolics, and fatty acid derivatives, like ephedrine, sesquiterpenes, valeranone, jatamansone, nardostachone, himachalene & cedrol which have been previously reported in phytochemical literature. HPTLC profiling suggested the presence of cedrol, himachalol, vasicinone, ephedrine, jatamansone, valerenic acid, etc. The combined application of GCMS and HPTLC demonstrates the presence of multiple phytoconstituents useful for analytical profiling, quality control and standardization of Devdarvyadi Dhoomavarti. Fume carbon content and volatile oil content which was calculated reflected the values under acceptable limits.