Quality Characterization and Bio accessibility Profiling of Sitadi Agada by diffusion method

Abstract

Sitadi Agada is a classical herbo-mineral formulation indicated in the management of Shankavisha. Scientific validation of its pharmaceutical characteristics and bio accessibility is necessary to substantiate its traditional therapeutic claims in emergency conditions. Aim: To evaluate the pharmaceutical parameters and in vitro bio accessibility of Sitadi Agada using the Franz diffusion cell method. Materials and Methods: Sitadi Agada was prepared using authenticated raw materials following modified classical procedures. The formulation was subjected to organoleptic evaluation, physicochemical analysis (loss on drying, ash values, extractive values, and pH), tablet evaluation (hardness, friability, and disintegration time), and microbiological testing as per standard guidelines. Bio accessibility was assessed using the Franz diffusion cell diffusion technique to study the release and diffusion profile of active constituents. Results: The formulation exhibited acceptable organoleptic characteristics with a sweet taste and pleasant odour. Physicochemical analysis revealed low moisture content (0.59%), acceptable ash values, higher water-soluble extractive value (31.42%) compared to alcohol-soluble extractive value, and near-neutral pH (6.8). Tablet evaluation showed rapid disintegration (3 min 30 s), adequate hardness (3.5 kg/cm²), and low friability (0.5%). The Franz diffusion study demonstrated effective diffusion of water-soluble phytoconstituents, indicating favourable bio accessibility. Conclusion: Sitadi Agada was found to be pharmaceutically stable, microbiologically safe, and bio accessible. The predominance of water-soluble constituents and rapid disintegration support its potential oral bioavailability. The study provides preliminary scientific evidence supporting the traditional use of Sitadi Agada in the management of Shankavisha (pseudo-snakebite) and fear-induced manifestations.